FluorCam 模(mo)塊(kuai)式(shi)植(zhi)物(wu)表型成(cheng)像(xiang)技(ji)術(shu)方(fang)案

FluorCam模(mo)塊(kuai)式(shi)植(zhi)物(wu)表型成(cheng)像(xiang)系統由(you)模(mo)塊(kuai)式(shi)光源板、葉綠(lv)素熒(ying)光成(cheng)像(xiang)與(yu)多(duo)光譜熒光成(cheng)像(xiang)鏡(jing)頭、選(xuan)配(pei)RGB成(cheng)像(xiang)、紅(hong)外(wai)熱成(cheng)像(xiang)及(ji)高(gao)光譜成(cheng)像(xiang)模(mo)塊(kuai)等組(zu)成(cheng)，廣(guang)泛應用(yong)於(yu)作物(wu)表型分析(xi)、植(zhi)物(wu)光合生(sheng)理研(yan)究(jiu)檢測、遺傳(chuan)育(yu)種(zhong)、植(zhi)物(wu)生(sheng)理生(sheng)態學(xue)研(yan)究(jiu)等。其主(zhu)要(yao)技(ji)術(shu)功能特點(dian)如(ru)下：

1.激發(fa)光源板、成(cheng)像(xiang)單(dan)元(yuan)等(deng)均采(cai)用(yong)模(mo)塊(kuai)式(shi)，配(pei)置靈活(huo)、高(gao)性(xing)價(jia)比、可(ke)擴(kuo)展性(xing)強(qiang)

2.標(biao)配(pei)葉綠素熒(ying)光成(cheng)像(xiang)和(he)多(duo)光譜熒光成(cheng)像(xiang)，成(cheng)像(xiang)面積(ji)20x20cm

3.可選(xuan)配(pei)不(bu)同(tong)激(ji)發(fa)光源，不(bu)同(tong)激(ji)發(fa)光源可隨意更換(huan)，以滿(man)足(zu)高(gao)等(deng)植(zhi)物(wu)、藻類(lei)及(ji)光生(sheng)物(wu)學(xue)研(yan)究(jiu)需求

4.可選(xuan)配(pei)GFP、YFP、DAPI等穩(wen)態熒(ying)光成(cheng)像(xiang)及(ji)活(huo)體(ti)熒(ying)光成(cheng)像(xiang)

5.可選(xuan)配(pei)PARabs、NDVI成(cheng)像(xiang)分(fen)析(xi)

6.可(ke)擴(kuo)展選(xuan)配(pei)RGB成(cheng)像(xiang)分(fen)析(xi)、紅(hong)外熱成(cheng)像(xiang)分(fen)析(xi)、VISIR高(gao)光譜成(cheng)像(xiang)分(fen)析(xi)模(mo)塊(kuai)

7.可(ke)選(xuan)配(pei)葉綠素熒(ying)光高(gao)光譜成(cheng)像(xiang)，光譜解碼(ma)葉綠素熒(ying)光

8.可配(pei)置實驗室臺式(shi)成(cheng)像(xiang)系統、大田(tian)或(huo)溫(wen)室移(yi)動(dong)式(shi)成(cheng)像(xiang)系統、PenoTron-PTS自(zi)動(dong)傳(chuan)送式(shi)成(cheng)像(xiang)分(fen)析(xi)系統等(deng)不(bu)同(tong)板式(shi)

9.可選(xuan)配(pei)葉面溫度(du)在線(xian)測量(liang)監測 

自(zi)左(zuo)至(zhi)右(you)依次為(wei)：FluorCam模(mo)塊(kuai)式(shi)葉(ye)綠(lv)素熒(ying)光成(cheng)像(xiang)系統（配(pei)備有紅外(wai)熱成(cheng)像(xiang)模(mo)塊(kuai)）、FluorCam-IQ移(yi)動(dong)式(shi)葉(ye)綠(lv)素熒(ying)光與(yu)高(gao)光譜成(cheng)像(xiang)系統、PhenoTron-PTS植(zhi)物(wu)表型成(cheng)像(xiang)分(fen)析(xi)系統（配(pei)備有葉綠(lv)素熒(ying)光成(cheng)像(xiang)、多(duo)光譜熒光成(cheng)像(xiang)、RGB成(cheng)像(xiang)及(ji)高(gao)光譜成(cheng)像(xiang)分(fen)析(xi)模(mo)塊(kuai)）

綜合(he)利(li)用(yong)FluorCam多光譜熒光成(cheng)像(xiang)、紅(hong)外(wai)熱成(cheng)像(xiang)及(ji)高(gao)光譜成(cheng)像(xiang)技(ji)術(shu)研(yan)究(jiu)評估(gu)油(you)菜(cai)在不(bu)同(tong)氣候變化條(tiao)件下的響(xiang)應機(ji)制(zhi)和健(jian)康（CCC：目前(qian)的氣候條(tiao)件不(bu)變，白天(tian)平(ping)均溫(wen)度(du)26度(du)、CO2濃(nong)度(du)408ppm；RCP4.5: 基於(yu)IPCC報(bao)告(gao)推(tui)測的2081–2100年氣候條(tiao)件（基於(yu)當(dang)前(qian)政府的(de)氣候變化應對(dui)政策(ce)，白天(tian)平(ping)均溫(wen)度(du)29度(du)、CO2濃(nong)度(du)650ppm）：RCP 8.5：基於(yu)IPCC報(bao)告(gao)推(tui)測的2081–2100年氣候條(tiao)件（不(bu)限制(zhi)溫室氣體排(pai)放(fang)，白天(tian)平(ping)均溫(wen)度(du)32度(du)、CO2濃(nong)度(du)1000ppm））。左(zuo)圖(tu)為(wei)RGB成(cheng)像(xiang)，中(zhong)圖(tu)為(wei)不(bu)同(tong)日期的F440、F520、F680/F740及(ji)葉片空(kong)氣溫度(du)差T_L-T_A，右(you)圖(tu)為(wei)不(bu)同(tong)日期的光譜曲線(xian)

左(zuo)圖(tu)與(yu)中(zhong)圖(tu)：西(xi)葫(hu)蘆病(bing)害(hai)感染(ran)多光譜熒光成(cheng)像(xiang)與(yu)紅(hong)外熱成(cheng)像(xiang)檢(jian)測；右(you)圖(tu)：葡(pu)萄(tao)卷葉(ye)病(bing)毒葉(ye)綠(lv)素熒(ying)光成(cheng)像(xiang)檢(jian)測

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Monica Pineda etc. Health Status of Oilseed Rape Plants Grown under Potential Future Climatic Conditions Assessed by Invasive and Non-Invasive Techniques. Agronomy, 2022